PittsburghV1, Main, Exploration, bibRecord, 003171

Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos.

Identifieur interne : 003171 ( Main/Exploration ); précédent : 003170; suivant : 003172

Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos.

Auteurs : Geon A. Kim [Corée du Sud] ; Hyun Ju Oh [Corée du Sud] ; Tae Hee Lee [États-Unis] ; Ji Hyun Lee [États-Unis] ; Sang Hwan Oh [États-Unis] ; Ju Hyun Lee [Canada] ; Jin Wook Kim [Corée du Sud] ; Se Woon Kim [États-Unis] ; Byeong Chun Lee [Corée du Sud]

Source :

Theriogenology [ 1879-3231 ] ; 2014.

RBID : pubmed:24157230

Descripteurs français

English descriptors

KwdEn :
- Adipocytes (cytology), Animals, Cell Culture Techniques (veterinary), Cell Differentiation, Cells, Cultured, Cloning, Organism (methods), Cloning, Organism (veterinary), Culture Media, Dogs, Embryonic Development, Gene Expression Profiling, Mesenchymal Stromal Cells (cytology), Nuclear Transfer Techniques (veterinary).
MESH :
- chemical : Culture Media.
- cytology : Adipocytes, Mesenchymal Stromal Cells.
- methods : Cloning, Organism.
- veterinary : Cell Culture Techniques, Cloning, Organism, Nuclear Transfer Techniques.
- Animals, Cell Differentiation, Cells, Cultured, Dogs, Embryonic Development, Gene Expression Profiling.

Abstract

Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures possess different capacities to support pre-implantation development of SCNT embryos. The aim of this study is to investigate whether two culture medium (RCMEP, Dulbecco's modified Eagle's medium [DMEM]) affect gene expression of ASCs, subsequent development of interspecies SCNT (iSCNT) and gene expression of cloned embryos. The RCMEP-cultured cells contained significantly greater amounts of SOX2, NANOG, OCT4, DNMT1, and MeCP2 than DMEM-cultured cells (P < 0.05). In iSCNT, the use of DMEM medium for culturing cells resulted in similar development to the blastocyst stage than those derived from RCMEP cultured cells (4.5% and 3.2%, respectively; P > 0.05). The expression of all transcripts except for DNMT1 in cloned blastocysts from RCMEP cultured cells followed those of cloned blastocysts derived from DMEM cultured cells. The alteration of gene expression in ASCs by culture medium was not manifested in the iSCNT embryos derived from these cells. Although the culture medium can induce changes of gene expression by ASCs, such alterations in donor cells did not affect the developmental competence or gene expression patterns of iSCNT embryos.

DOI: 10.1016/j.theriogenology.2013.09.018
PubMed: 24157230

Affiliations:

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adipocytes (cytology)</term>
<term>Animals</term>
<term>Cell Culture Techniques (veterinary)</term>
<term>Cell Differentiation</term>
<term>Cells, Cultured</term>
<term>Cloning, Organism (methods)</term>
<term>Cloning, Organism (veterinary)</term>
<term>Culture Media</term>
<term>Dogs</term>
<term>Embryonic Development</term>
<term>Gene Expression Profiling</term>
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<term>Nuclear Transfer Techniques (veterinary)</term>
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<term>Analyse de profil d'expression de gènes</term>
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<term>Cellules cultivées</term>
<term>Cellules stromales mésenchymateuses (cytologie)</term>
<term>Chiens</term>
<term>Clonage d'organisme ()</term>
<term>Clonage d'organisme (médecine vétérinaire)</term>
<term>Différenciation cellulaire</term>
<term>Développement embryonnaire</term>
<term>Milieux de culture</term>
<term>Techniques de culture cellulaire (médecine vétérinaire)</term>
<term>Techniques de transfert nucléaire (médecine vétérinaire)</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term>
</keywords>
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<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Clonage d'organisme</term>
<term>Techniques de culture cellulaire</term>
<term>Techniques de transfert nucléaire</term>
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<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Cell Culture Techniques</term>
<term>Cloning, Organism</term>
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<term>Cellules cultivées</term>
<term>Chiens</term>
<term>Clonage d'organisme</term>
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<front><div type="abstract" xml:lang="en">Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures possess different capacities to support pre-implantation development of SCNT embryos. The aim of this study is to investigate whether two culture medium (RCMEP, Dulbecco's modified Eagle's medium [DMEM]) affect gene expression of ASCs, subsequent development of interspecies SCNT (iSCNT) and gene expression of cloned embryos. The RCMEP-cultured cells contained significantly greater amounts of SOX2, NANOG, OCT4, DNMT1, and MeCP2 than DMEM-cultured cells (P < 0.05). In iSCNT, the use of DMEM medium for culturing cells resulted in similar development to the blastocyst stage than those derived from RCMEP cultured cells (4.5% and 3.2%, respectively; P > 0.05). The expression of all transcripts except for DNMT1 in cloned blastocysts from RCMEP cultured cells followed those of cloned blastocysts derived from DMEM cultured cells. The alteration of gene expression in ASCs by culture medium was not manifested in the iSCNT embryos derived from these cells. Although the culture medium can induce changes of gene expression by ASCs, such alterations in donor cells did not affect the developmental competence or gene expression patterns of iSCNT embryos.</div>
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Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos.

Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos.

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